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KMID : 0357920100440040376
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Korean Journal of Pathology
2010 Volume.44 No. 4 p.376 ~ p.383
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Pathologic Characteristics of Ovarian Hemorrhagic Polycyst in Estrogen Receptor-alpha (ERα) Knockout Mice and Roles of ERα in Hemorrhagic Polycyst
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Son Hyun-Jin
Kim Ju-Hun
Lee Hye-Kyung
Park Mi-Ja
Kang Dong-Wook
Ko Che-Myong
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Abstract
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Background: Polycystic ovary syndrome (PCOS) is the most common endocrinopathy causing anovulation in women of childbearing age. It has been well established that estrogen receptor-alpha knockout (ERαKO) mice display several pathologic ovarian phenotypes of PCOS. The aims of this study were to determine ovarian pathology in new ERαKO mice using a CreloxP approach and intra-ovarian ERα function as regulating key aspects of PCOS.
Methods: ERαKO mice, which were deficient in exon 3 of the ERα gene, were used. Immunohistochemical studies were done on ovaries of control and ERαKO mice using antibodies specific to ERα, ERβ, inhibin-α, and α-smooth muscle actin (SMA), as well as histochemical staining using Sudan black-B.
Results: All ovaries of ERαKO mice were larger than control mouse ovaries and displayed a disrupted theca-interstitial tissue organization, multiple atretic follicles and multiple hemorrhagic cysts. None of the ERαKO mouse ovaries showed a corpus luteum. In addition, heavy deposition of Sudan black-B positive foamy cells was seen. The theca externa of preantral immature follicles and hemorrhagic cysts showed strong expression of α-SMA.
Conclusions: ERαKO mice show hemorrhagic polycystic ovaries and hyperplasia of the theca externa. This study demonstrates that the ERα is the functional key to the pathogenesis of PCOS.
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KEYWORD
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Polycystic ovary syndrome, Estrogen receptor-alpha knockout (ERαKO), Hemorrhagic polycysts, Immunohistochemistry
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